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Product Info Summary
| SKU: | P00026-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00026-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody catalog # P00026-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EAC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-c-Myc (S62)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00026-2 is reactive to MYC in Human, Mouse, Rat
Applications
P00026-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
48.804kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 100-1:500
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody .
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-c-Myc (S62) expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)
Gene Name
MYC
Full Name
Myc proto-oncogene protein
Weight
48.804kDa
Alternative Names
9E10 Myc; 9E10 Myc; avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYC, check out the MYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (P00026-2)
Hello CJ!
P00026-2 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Constitutive %u03B2-catenin activation in osteoblasts impairs terminal osteoblast differentiation and bone quality
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Metformin Inhibits Tumorigenesis and Tumor Growth of Breast Cancer Cells by Upregulating miR-200c but Downregulating AKT2 Expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Zhang D, Fei F, Li S, Zhao Y, Yang Z, Qu J, Zhang X, Yin Y, Zhang S. J Cancer. 2017 Jul 5;8(11):2114-2123. doi: 10.7150/jca.19723. eCollection 2017. The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody
Question
I would like using your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody for chromosome organization studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thanks for your inquiry. This P00026-2 anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody P00026-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
A. Collins
Verified customer
Asked: 2017-09-21
Answer
The anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody (P00026-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
Question
We ordered your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody for IP on uterus in the past. I am using human, and We are going to use the antibody for WB next. My lab would like examining uterus as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
I looked at the website and datasheets of our anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody and I see that P00026-2 has been validated on human in both IP and WB. Thus P00026-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-08
Question
We have been able to see staining in mouse cervix carcinoma. Are there any suggestions? Is anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
C. Miller
Verified customer
Asked: 2016-11-28
Answer
From literature cervix carcinoma does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-11-28
Question
My boss were content with the WB result of your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody. However we have seen positive staining in cervix nucleus using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
A. Baker
Verified customer
Asked: 2013-03-05
Answer
From literature, cervix does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-03-05
